TABLE 2.

Cytoplasmic proteins identified on 2D gels of S. aureus

ProteinFunctionLocus in S. aureus COL
Induced by a hemB mutation
    AdhEnergy metabolism: fermentation (alcohol dehydrogenase)SA0660
    ArcAEnergy metabolism: amino acids and amines (arginine deiminase)SA2657
    ArcBEnergy metabolism: amino acids and amines (ornithine carbamoyltransferase)SA2656
    ClpLATP-dependent Clp proteinase chain ClpLSA2563
    EnoEnergy metabolism: glycolysis/gluconeogenesis (enolase)SA0842
    FbaEnergy metabolism: glycolysis/gluconeogenesis (fructose bisphosphate aldolase, class II)SA2117
    FdaEnergy metabolism: glycolysis/gluconeogenesis (fructose bisphosphate aldolase, class I)SA2622
    GapEnergy metabolism: glycolysis (glyceraldehyde-3-phosphate dehydrogenase)SA0838
    HmpUnknown function: general (flavohemoprotein, putative)SA0220
    l-LdhEnergy metabolism: fermentation (l-lactate dehydrogenase)SA0222
    PgiEnergy metabolism: glycolysis (phosphoglucomutase)SA0966
    PgkEnergy metabolism: glycolysis (phosphoglycerate kinase)SA0839
    PflEnergy metabolism: fermentation (pyruvate formate lyase)SA0205
    PykEnergy metabolism: glycolysis (pyruvate kinase)SA1745
    TpiEnergy metabolism: glycolysis (triose phosphate isomerase)SA0840
Not influenced by a hemB mutation
    PdhBEnergy metabolism: pyruvate dehydrogenase (pyruvate dehydrogenase complex, E1 component; pyruvate dehydrogenase, beta subunit)SA1103
    PdhDEnergy metabolism: pyruvate dehydrogenase (pyruvate dehydrogenase complex, E3 component; lipoamide dehydrogenase)SA1105