TABLE 2.

Primers and conditions used to confirm in-frame deletions

DeletionPrimersConditions
sopB5′-CGGGATCCGCGTTACGCAATCACTATC33 cycles of 94°C for 30 s, 52°C for 30 s, and 72°C for 5 min
5′-GCGGCCGCCCGTTGACATCCTCCAGAA
sopE5′-CGGGATCCTCTTGGCGCGTAGTCCTTC33 cycles of 94°C for 30 s, 52°C for 30 s, and 72°C for 4.5 min
5′-TTTGCGGCCGCGCACTGGATACGCTGAACGA
sopE25′-CGGGATCCGCGCAGGCGTTTAGAAGACAGTT33 cycles of 94°C for 30 s, 54°C for 30 s, and 72°C for 5 min
5′-GCGGCCGCGTTCCAGCATCAGCCACTTG
sipA5′-GCGGCCGCACCTGGGGTTGAGTCCTAC33 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 6 min
5′-CCCGGGACACCAAGGCACGAG
invB5′-GGGCGCCAAGAGAAAAAGATGTC33 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1.75 min
5′-CAGGGGTAAAACGCTCAACGATT