TABLE 3.

Comparisons of lasB, aprA and lasR transcript levels in early log phase (120 min) and stationary phase (300 min) for P. aeruginosa strains

Strain no. and originaAmt of transcript at indicated culture timebComparison with lasR gene of PAO1
lasR transcriptlasB transcriptaprA transcript
120 min300 min120 min300 min120 min300 min
47, B0.241.940.043.461.7197.93Full identity
63, E0.244.090.61.740.093.23C201 stopc
41, B0.450.660.040.011.036.93Full identity
45, B0.594.141.5113.410.170.57Silent mutations
53, B0.8810.361.431.444.512.91Insert
42, B0.921.672.584.380.131Q186P
58, E1.092.590.2718.940.111.78Silent mutations
55, B1.3617.660.830.461.833.11Full identity
43, B1.647.770.160.170.231.81A70E
37, B2.268.091.5920.922.8564.95Silent mutations
30, P2.429.110.4612.140.2929.62Silent mutations
67, E2.763.211.270.581.083.64Full identity
15, P2.965.580.635.981.42309.28Silent mutations
51, B3.197.382.473.3515.5917.21Full identity
66, E3.626.493.022.635.099.56Insertions
24, P3.9344.010.8727.610.88111.67Silent mutations
48, B4.025.1615.059.862.141.72F210L
16, P4.8312.930.254.740.8644.89Full identity
39, B5.1527.220.967.730.8821.5Silent mutations
11, P6.8627.990.495.070.2933.74Multiple substitutionsd
64, E7.4528.911.87201.684.51144.01Silent mutations
23, P9.5488.213.0187.954.25141.91Silent mutations
71, E11.3512.940.56.360.3435.45Multiple substitutions
20, P11.556.22.590.616.952.01Full identity
50, B12.6726.982.2742.860.41134.41K25M
65, E14.770.881.7121.083.38361.46Full identity
69, E15.2419.477.51119.057.69197.64Silent mutations
36, P15.8433.053.060.440.454.78Insertions
19, P18.5744.8810.018.843.3131.67A50G
38, B21.24351.73155.552.12203.25Silent mutations
9, P22.1551.882.3273.681.223.24Full identity
14, P34.2737.361.533.140.949.12Full identity
13, P37.2125.972.4977.860.359.51V226I
61, E42.1137.974.5758.776.48133.12Full identity
27, P106.29414.395.02258.0516.84269.79Silent mutations
PAO18.3912.238.22129.075.12210.15Full identity
  • a B, strain isolated from patient with bacteremia; P, strain isolated from patient with pneumonia; E, strain isolated from the environment.

  • b lasB, aprA, and lasR transcript levels were normalized by using the ratio of the amount of these transcripts to the small-subunit rRNA level and multiplying by 1,000.

  • c Amino acid substitution.

  • d Strains 11 and 71 had the following amino acid substitutions: R66K, N136S, A137N, and G172N.