TABLE 2.

Comparison of the lasR sequence of 66 P. aeruginosa strains and a subset of 35 strains with the internal fragment of the lasR gene of PAO1b

Identity or differenceOrigin of strain
Pneumoniaa (n= 30)Bacteremiaa (n= 20)Environmenta (n = 16)
Full identity
    All strains15 (50)8 (40)7 (44)
    RT-PCR strains4 (13)4 (20)3 (19)
Silent mutations
    All strains11 (37)7 (35)6 (38)
    RT-PCR strains4 (13)4 (20)3 (19)
Nucleotide changes resulting in amino acid changes
    All strains3 (10)4 (20)2 (12)
    RT-PCR strains4 (13)4 (20)2 (12)
Insertions
    All strains1 (3)1 (5)1 (6)
    RT-PCR strains1 (3)1 (5)1 (6)
  • a The numbers in parentheses indicate the percentages of lasR sequence retrieved from all strains from each origin.

  • b The strains were studied by using real-time PCR (RT-PCR). The internal fragment used for comparison was 665 bp; the lasR gene of PAO1 is 720 bp.