TABLE 2.

Structural parameters of developing forespore peptidoglycana

Strain% of spore PG made% of muramic acid with side chains of:% of muramic acid with cross-link
δ-LactamAlaTriPTP
Wild type531.437.024.47.16.5
1946.641.35.86.33.7
4946.632.14.716.63.4
8448.327.72.721.33.6
10049.323.42.025.23.5
ΔybaK::Spr432.542.420.64.54.5
1539.342.314.24.23.8
4245.243.05.95.82.4
7645.529.85.019.73.3
10048.424.72.424.53.4
In-frame ΔybaK230.640.122.17.225.4
1444.942.48.04.72.6
5844.830.35.519.43.0
8147.029.63.320.02.8
10048.624.92.024.63.0
Wild type + pSpac-ybaK-cwlD544.531.16.917.51.6
5045.630.86.517.12.2
8548.629.93.418.02.5
10050.717.31.430.63.3
Δ(ybaK-cwlD)::Spr + pSpac-ybaK-cwlD1030.942.77.419.12.4
4836.439.35.319.02.0
8237.333.64.424.73.3
10039.620.72.137.64.7
Δ(ybaK-cwlD)::Spr + amyE::(dacFp-cwlD) (forespore expression of cwlD)77.023.530.538.912.3
2310.233.814.341.85.8
5213.4637.078.2241.244.6
8416.037.46.739.95.3
10019.728.93.148.35.8
Δ(ybaK-cwlD)::Spr + amyE::(dacBp-cwlD) (mother cell expression of cwlD)84.927.342.825.17.9
1940.029.612.817.63.4
4845.129.05.820.22.9
7947.027.94.221.02.7
10049.419.62.228.93.1
  • a Abbreviations: TriP, tripeptide; TP, tetrapeptide; PG, peptidoglycan.