TABLE 4.

Annotation of all ORFs located within integron TNCP23

Gene identificationCoordinatesDirectionLength (aa)aGC content (%)Gene nameAnnotation result and homologous gene productsBLAST search resultbE value of best BLAST hit
LeftRight
TNCP16385726461.01tnpTransposase; similar to Tnp from S. enterica subsp. enterica serovar Typhimurium; IS6100 elementAAG03007E-112
TNCP21.0231.3109564.31Hypothetical protein similar to ORF6 (P. aeruginosa)AAK963998E-33
TNCP31.3341.83416666.27Acetyltransferase similar to ORF5 (P. aeruginosa)CAA114781E-90
TNCP41.9622.80127961.57sulIDihydropteroate synthase Sul1 (P. aeruginosa); sulfonamide resistance proteinAAA25859E-143
TNCP52.7953.14211550.00qacEdeEthidium bromide resistance proteinAAC443161E-42
ltaQacEdelta1 (P. aeruginosa)
TNCP63.2994.04824951.76aadBAminoglycoside adenylyltransferase AadB (P. aeruginosa); streptothricin-spectinomycin resistance proteinAAD399347E-97
TNCP7a3.9784.93731961.25int1Phage integrase similar to Int from P. aeruginosaAAA25857E-154
TNCP7b4.0684.37610262.35Hypothetical protein similar to ORF2 (E. coli)B268393E-47
TNCP85.0545.59618063.17ISxac3 transposase similar to XACa0010 (Xanthomonas axonopodis pv. citri)NP_6446961E-18
TNCP95.6025.99713162.37Conserved hypothetical protein similar to XCC1632 (Xanthomonas campestris pv. campestris)NP_6370024E-40
TNCP105.9946.2458361.51Conserved hypothetical protein similar to XCC1631 (X. campestris pv. campestris)NP_6370011E-35
TNCP116.3106.99322764.77Putative invertase/recombinase (X. axonopodis)NP_6446922E-75
TNCP127.1137.96428369.25Putative resolvase similar to pVS1 resolvaseAAD196762E-80
TNCP138.0458.67721063.98parAParA partitioning protein (Serratia marcescens)BAB719509E-92
TNCP148.6988.9197360.36parBPutative ParB plasmid stabilization protein (Pseudomonas alcaligenes)AAD403350.006
TNCP158.9729.98533763.91repARepA replication protein (X. axonopodis pv. citri)NP_642756E-110
TNCP1610.90011.79029670.82kfrAKfrA protein (X. axonopodis pv. citri)NP_6447282E-12
TNCP1711.96412.35613069.47No significant similarity
TNCP1812.36714.88984025.68traGPlasmid transfer factor protein similar to TraG (M. loti)BAB549198E-36
TNCP1914.92415.1457359.46traDPutative conjugative protein similar to TraD (Rhizobium sp.)NP_4438300.22
TNCP2015.16815.4258555.81traCConjugal transfer protein similar to TraC (Brucella suis 1330)AE0145361E-12
TNCP2115.44218.9631,17318.26traAConjugal transfer protein similar to TraA (M. loti); MobA/MobL family conserved domainBAB52121E-132
TNCP22a19.14519.71719063.70Similar to truncated N-terminal conserved domain of phage integrases
TNCP22b19.51119.7297263.01No significant similarity, overlapping by TNCP22a
TNCP2319.71620.65131168.27Serine protease similar to Ycg4E (C. glutamicum)AAG002854E-74
TNCP2420.74721.08511266.67Hypothetical protein similar to coenzyme F390 synthetase XF1916 (Xylella fastidiosa)NP_2992020.16
TNCP2521.14821.3546866.18Hypothetical protein similar to XfasO0625 (X. fastidiosa)ZP_00040826E-6
TNCP2621.35121.5245771.84No significant similarity
TNCP2721.52122.35727863.08Putative type II restriction enzyme similar to XfasA1931 (X. fastidiosa Dixon)ZP_00039987E-104
TNCP2822.28423.03024860.78tnpTruncated transposase; similar to Tnp from S. enterica subsp. enterica serovar Typhimurium IS6100 elementAAG03007E-112
  • a aa, amino acids.

  • b GenBank accession number.