TABLE 1.

B. subtilis strains used

StrainGenotypea (references)Transformation
DonorRecipient
DPVB3ΔybaK::SppDPV5PS832
DPVB19Δ(ybaK-cwlD)::SppDPV15PS832
DPVB47In-frame ΔybaKpDPV20PS832
DPVB70cwlD-lacZ::CmpDPV75PS832
DPVB71ΔybaK::Sp cwlD-lacZ::CmPDPV75DPVB3
DPVB72In-frame ΔybaK cwlD-lacZ::CmpDPV75DPVB47
DPVB75(pBL1 lacI Erm) (20)pBL1PS832
DPVB76Δ(ybaK-cwlD)::Sp(pBL1 lacI Erm)pBL1DPVB19
DPVB77Δ(ybaK-cwlD)::Sp(pSpac-ybaK-cwlD Kn)(pBL1 lacI Erm)pDPV36DPVB76
DPVB100(pSpac-ybaK-cwlD, Kn)(pBL1 lacI Erm)pDPV36DPVB75
DPVB205Δ(ybaK-cwlD)::Sp amyE::dacFp-cwlD::CmpDPV123DPVB19
DPVB206Δ(ybaK-cwlD)::Sp amyE::dacBp-cwlD::CmpDPV124DPVB19
PS832Prototrophic revertant of strain 168 (lab stock)
PS2307cwlD::Cm (27, 30)
  • a Abbreviations: Sp, resistance to spectinomycin; Cm, resistance to chloramphenicol; Erm, resistance to erythromycin and lincomycin; Kn, resistance to kanamycin.