TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)aSource or reference
Bacteroides strains
    B. thetaiotaomicron BT5482Rfr15
    B. thetaiotaomicron BT5482::341Rfr Tcr; BT5482 containing CTn341 from mating with CLA341This work
    B. fragilis ADB77Rfr Tpr ΔthyA1
    B. fragilis ADB77::Tn4555Rfr Tpr Fxr ΔthyAThis work
    B. vulgatus CLA341Tcr Fxr; clinical strain containing CTn341 and Tn455510
E. coli strains
    EC100Str F mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ rpsL nupGEpicentre, Madison Wis.
    HB101::RK231Kmr Tcr Str; HB101 containing RK2315
Plasmids
    pFD516Spr Emr; 7.7-kb suicide vector19
    pYT102Cmr Tcr; 8.2 kb, containing the thyA gene1
    pFD851Apr Fxr; 10.7-kb shuttle vectorThis work
    pFD852Apr Fxr; 12.1-kb derivative of pFD851 containing the xis geneThis work
    pFD859Apr Fxr; 12.1-kb derivative of pFD851 containing the tnpC geneThis work
    pFD861Apr Fxr; 13-kb derivative of pFD851 containing the tnpC xis genesThis work
  • a Antibiotic resistance phenotypes at the following concentrations: Rf, rifampin (20 μg/ml); Tc, tetracycline (5 μg/ml); Tp, trimethoprim (100 μg/ml); Fx, cefoxitin (20 μg/ml); St, streptomycin (25 μg/ml); Sp, spectinomycin (40 μg/ml); Cm, chloramphenicol (25 μg/ml); Ap, ampicillin (50 μg/ml); Em, erythromycin (15 μg/ml).