TABLE 3.

Annotation of all ORFs located within pKLC102

Gene identificationCoordinatesDirectionLength (aa)aGC content (%)Gene nameAnnotation result and homologous gene productsBLAST search resultbE value of best BLAST hit
LeftRight
CP111399729460.23sojSimilar to chromosome partitioning-related protein (Xylella fastidiosa 9a5c)NP_2990736e-67
CP2999172424159.78Similar to hypothetical protein from Pseudomonas resinovoransNP_7587062e-25
CP31.7212.21816562.25Hypothetical protein similar to PA2226 (P. aeruginosa)NP_2509165E-27
CP42.2242.97024861.85Hypothetical protein similar to unknown protein encoded by gene Z2097 within prophage CP-933O (E. coli O157:H7 EDL933)NP_2875556E-10
CP52.9673.65322864.05Hypothetical protein similar to Magn3145 (Magnetospirillum magnetotacticum)ZP_000504891E-10
CP63.6534.35423364.53Hypothetical protein similar to an intragenic region upstream of Psyr3996 (P. syringae pv. syringae B728a)NZ_AABP020000092E-61
CP74.3515.05823558.62Hypothetical protein homologous to EsV-1-119 ORF119 (Ectocarpus siliculosus virus)NP_0776040.039
CP85.0425.2607257.08No significant similarity
CP95.3236.60042561.11dnaBReplicative DNA helicase PA4931 (dnaB) (P. aeruginosa)NP_253618E-122
CP107.0397.39511855.74No significant similarity
CP117.8388.36517561.74No significant similarity
CP128.3628.6198560.08No significant similarity
CP138.6129.10916562.85No significant similarity
CP149.1029.3357766.24Conserved hypothetical protein similar to Bcep0486 (B. fungorum)ZP_000277181E-06
CP159.33510.35133864.01Conserved hypothetical protein homologous to PA3849 (P. aeruginosa) and to a nucleoid associated protein (E. coli O157:H7)NP_252538E-175
CP1610.34810.6028458.43Putative DNA-binding protein homologous to PA3385 (AlgZ) (P. aeruginosa)AAD553640.028
CP1710.59912.33257758.36Conserved hypothetical protein similar to ORF SG102 (P. aeruginosa); ParB-like nuclease domainAAN62323E-104
CP1812.36013.11525159.13Hypothetical protein Bcep0488 (B. fungorum)ZP_000277201E-30
CP1915.76616.49424263.24Conserved hypothetical protein similar to ORF C104 (P. aeruginosa)AAN621974E-64
CP2016.50017.04818260.84Conserved hypothetical protein similar to ORF C103 (P. aeruginosa)AAN621961E-24
CP2117.09517.93427960.83Putative antirepressor homologous to sb41 (Salmonella enterica serovar Typhimurium phage ST64B)NP_7004146E-23
CP2217.96418.45216262.17ssbPutative single-stranded DNA-binding protein homologous to ssb (P. aeruginosa); single-strand binding conserved domainAAN623185E-30
CP2318.87119.1529353.90No significant similarity
CP2419.28919.5498652.87No significant similarity
CP2519.57119.98113660.83Putative nucleic acid binding protein; contains PIN conserved domain (similarity with an analogous gene from Salmonella enterica serovar Typhimurium LT2)NP_4619502E-51
CP2619.98120.2117657.57Putative virulence-associated protein similar to cytoplasmic protein from Salmonella enterica serovar Typhimurium LT2; VagC conserved domainNP_4905912E-20
CP2720.46722.38663962.19topATopoisomerase I; similar to XFa0003 (X. fastidiosa)NP_061659E-148
CP2822.69422.9036951.90cspACold acclimation protein similar to PA3266 (CspA) (P. aeruginosa)NP_2519562E-18
CP2923.12425.01362960.37No significant similarity
CP3025.01026.98365764.18Putative helicase similar to Psyr4003 (P. syringae pv. syringae); SNF2 family N-terminal domain; DEAD-like helicase superfamily; helicase superfamily C-terminal domainZP_001276830.0
CP3127.10527.88726046.23Putative ABC transporter similar to mlr7981 (Mesorhizobium loti); ATP-binding proteinBAB536389E-85
CP3227.92029.17341741.55Hypothetical protein similar to SMa2241 (S. meliloti plasmid pSymA)NP_436454E-126
CP3329.34730.47137465.16pilLPilL (Yersinia pseudotuberculosis)AAL054163E-36/PICK>
CP3430.47132.18056963.86pilNPilN (E. coli)AAL055188E-56
CP3532.29833.50940364.03pilOPilO (Salmonella enterica serovar Typhi)AAF148155E-13
CP3633.49934.03217770.97pilPPilP (Salmonella enterica serovar Typhimurium plasmid R64)BAA779762E-04
CP3734.04135.62152663.25pilQPilQ (S. enterica subsp. enterica serovar Dublin)AAF812137E-74
CP3835.62136.70035963.80pilRPilR (Salmonella enterica serovar Typhi)AAC988862E-40
CP3936.77937.25215760.13pilSPilS; prepilin similar to hypothetical protein from P. syringae pv. syringae B728a and to type IV prepilin (Y. pseudotuberculosis)ZP_001276903E-18
CP4037.24938.19031366.03pilUPilU; prepilin peptidase (P. aeruginosa)S547021E-15
CP4138.19539.52344263.81pilVPilV; pilus tip adhesin (E. coli)CAA440996E-48
CP4239.55239.98914568.26pilMPilM (Salmonella enterica serovar Typhimurium plasmid R64)BAA779731.1
CP4340.91141.30012960.00No significant similarity
CP4441.37341.5525953.33No significant similarity
CP4541.55441.6884458.52No significant similarity
CP4642.01242.49115957.29Conserved hypothetical protein (P. fluorescens)ZP_000878944E-46
CP4742.86143.0586553.54Conserved hypothetical protein (S. enterica subsp. enterica serovar Typhi)NP_4586261E-10
CP4843.16343.4419250.18No significant similarity
CP4943.54444.25123552.68Conserved hypothetical protein similar to Pflu5179 (P. fluorescens)ZP_000878921E-41
CP5044.48744.83711663.25No significant similarity
CP5144.89445.68526361.62Conserved hypothetical protein similar to XF1760 (X. fastidiosa)NP_2990492E-42
CP5245.78846.15012058.13Conserved hypothetical protein similar to ORF C77 (P. aeruginosa)AAN621719E-21
CP5346.21846.4728461.18Conserved hypothetical protein similar to XAC2240 (Xanthomonas axonopodis pv. citri)NP_6425572E-08
CP5446.56447.16920164.03Conserved hypothetical protein similar to XAC2241 (X. axonopodis pv. citri 306)NP_6425581E-50
CP5547.19948.64448163.69Hypothetical protein similar to Bcep3609 (B. fungorum),ZP_00030785E-133
CP5648.74350.99875163.25Putative helicase similar to hypothetical protein Psyr4040 (P. syringae pv. syringae); SNF2 family N-terminal domain; DEAD-like helicase superfamily; helicase superfamily C-terminal domainZP_001277170.0
CP5751.57952.36426155.47Putative polyketide synthase similar to XF2135 (X. fastidiosa); HCCA isomerase conserved domainNP_2994148E-23
CP5852.30352.64411358.48Conserved hypothetical protein similar to Bcep7601 (B. fungorum); carboxymuconolactone decarboxylase conserved domainZP_000347062E-15
CP5952.73953.29918654.55Putative TetR family transcription regulator protein similar to ORF43 (P. putida plasmid pWWO); TetR conserved domainNP_5428323E-17
CP6053.36254.14726158.78Putative reductase protein similar to ORF44 (P. putida plasmid pWWO); short-chain dehydrogenase conserved domainNP_5428333E-76
CP6154.30354.71613759.66Putative TetR family transcription regulator protein similar to MA2493 (Methanosarcina acetivorans)NP_6173990.12
CP62a54.70955.56028357.51Phage integrase similar to hypothetical protein Reut5633 (R. metallidurans); phage integrase domainZP_000266071E-23
CP62b54.90455.40716759.52No significant similarity; inner ORF of the phage integrase CP62a
CP6356.33157.02323063.78Conserved hypothetical protein similar to Psyr4041 (P. syringae pv. syringae)ZP_001277182E-26
CP6457.03457.78925165.21Conserved hypothetical protein similar to Psyr4042 (P. syringae pv. syringae)ZP_001277195E-53
CP6557.77458.35519365.64Hypothetical protein similar to ORF SG70 (P. aeruginosa)AAN621615E-35
CP6658.35258.85216666.27Hypothetical protein similar to ORF SG69 (P. aeruginosa)AAN622917E-31
CP6759.13461.36574365.10Putative conjugation protein TraG similar to hypothetical protein Psyr4044 (P. syringae pv. syringae); TraG/TraD family conserved domainZP_001277210.0
CP6861.36562.11124861.18Conserved hypothetical protein similar to ORF SG67 (P. aeruginosa)AAN622895E-52
CP6962.12263.60349359.85Putative helicase similar to hypothetical protein Psyr4046 (P. syringae pv. syringae); UvrD helicase conserved domainZP_001277230.0
CP7063.73564.84436946.58Hypothetical protein similar to a probable RND efflux transporter mll1426 (M. loti)NP_1030184.4
CP7164.88965.20010349.68Conserved hypothetical protein similar to PA0713 (P. aeruginosa)NP_2494045E-16
CP7265.37365.6729957.00No significant similarity
CP7365.88366.1047367.57Hypothetical protein similar to Avin1322 (A. vinelandii)ZP_000896503E-24
CP7466.10766.3407764.53Hypothetical protein similar to Avin1321 (A. vinelandii)ZP_000896492E-8
CP7566.35866.71411857.98Hypothetical protein similar to Avin1320 (A. vinelandii)ZP_000896483E-15
CP7666.72567.11112866.93Hypothetical protein similar to Avin1319 (A. vinelandii)ZP_000896471E-32
CP7767.10867.76721961.06Hypothetical protein similar to Avin1318 (A. vinelandii)ZP_000896464E-78
CP7867.76468.64829466.33Hypothetical protein similar to Avin1317 (A. vinelandii)ZP_000896455E-88
CP7968.71070.13747564.15Hypothetical protein similar to Avin1316 (A. vinelandii)ZP_00089644E-149
CP8070.21170.55811564.94Hypothetical protein similar to Avin1315 (A. vinelandii)ZP_000896431E-47
CP8170.55873.50098063.00Hypothetical protein similar to Avin1314 (A. vinelandii)ZP_000896420.0
CP8273.49773.7819458.25Hypothetical protein similar to Avin1313 (A. vinelandii)ZP_000896412E-19
CP8373.77874.43421862.25Conserved hypothetical outer membrane protein similar to PA0982 (P. aeruginosa)NP_2496733E-87
CP8475.20876.39539562.88Putative flavoprotein reductase similar to hypothetical protein PA2566 (P. aeruginosa)NP_2512560.0
CP8576.41876.84014066.43Hypothetical protein similar to PA2565 (P. aeruginosa)NP_2512555E-25
CP8676.83077.69628863.78cheRCheR; trans-aconitate 2-methyltransferase similar to PA2564 (P. aeruginosa)NP_251254E-126
CP8777.83478.26514361.81Conserved hypothetical protein similar to Avin1308 (A. vinelandii)ZP_000896376E-20
CP8878.26579.20331264.86Hypothetical protein similar to Avin1307 (A. vinelandii)ZP-00089636E-123
CP8979.03880.60352164.56Putative integral membrane protein similar to ORF C42 (P. aeruginosa)AAN62272E-122
CP9080.60380.95011562.64Hypothetical protein similar to Avin1305 (A. vinelandii)ZP_000896340.001
CP9180.94782.48251158.72Hypothetical protein similar to Avin1304 (A. vinelandii)ZP_000896330.0
CP9282.92483.25611060.61Hypothetical protein (P. fluorescens); conserved domain of NikR transcriptional regulatorZP_000867168E-16
CP9383.26083.61011662.68Conserved hypothetical protein similar to AGR_C_2415 (A. tumefaciens strain C58; Cereon); ParE conserved domain of plasmid stabilization system proteinG975185E-15
CP9483.98892.5012,83765.32chvBBeta (1->2) glucan biosynthesis protein similar to ChvB (A. tumefaciens)NP_5333950.0
CP9593.34393.5677461.33Hypothetical protein similar to Pflu4565 (P. fluorescens)ZP_000872890.13
CP9693.78294.50123961.25Transcriptional regulator-related protein similar to SO2551 (Shewanella oneidensis); helix-turn-helix and cAMP regulatory protein conserved domainsNP_7181375E-78
CP9794.57294.93412060.33No significant similarity
CP9894.85295.53222664.02Hypothetical protein similar to XF0240 (X. fastidiosa)NP_2975337.1
CP9995.64197.65967262.51Fusion protein; putative fatty acid synthase similar to cyclopropane-fatty-acyl-2 phospholipid synthase mll4091 (M. loti)BAB508313E-67
CP10097.81599.00839761.81Probable fatty acid desaturase similar to PA0286 (P. aeruginosa)AAG03675E-124
CP10199.39699.75211845.94Hypothetical protein similar to Avin0596 (A. vinelandii)ZP_000889363E-21
CP10299.964101.88363958.70Conserved hypothetical protein; putative conjugative relaxase Tral; similar to hypothetical protein Avin0927 (A. vinelandii)ZP_000892600.0
CP103a101.880103.16342760.00xerCPhage integrase similar to XerC (P. syringae)AAM77365E-101
CP103b101.970102.47616860.36No significant similarity; inner ORF of phage integrase CP103a
  • a aa, amino acids.

  • b GenBank accession number.