TABLE 1.

B. subtilis strains used in this study

StrainGenotypeaSource
168 trpC2 BGSCb
BG4233ΔmtrB argC420
CYBS400::pJS648 amyE::PtrpPtrpP′-′lacZ Cmr27
CYBS306 mtrBΩTc amyE::PtrptrpE′-′lacZ Cmr24
PGBS11 argC4 amyE::PpabpabB-pabA′-′lacZ Cmr41
PLBS338PrototrophThis study
PLBS339 amyE::PtrpPtrpP′-′lacZ CmrThis study
PLBS340 amyE::PpabpabB-pabA′-′lacZ CmrThis study
PLBS341ΔmtrB amyE::PtrpPtrpP′-′lacZ CmrThis study
PLBS342ΔmtrB amyE::PpabpabB-pabA′-′lacZ CmrThis study
PLBS343 rho::neo (Kmr) amyE::PtrpPtrpP′-′lacZ CmrThis study
PLBS344 rho::neo (Kmr) amyE::PpabpabB-pabA′-′lacZ CmrThis study
PLBS345ΔmtrB rho::neo amyE::PtrpPtrpP′-′lacZ CmrThis study
PLBS346ΔmtrB rho::neo amyE::PpabpabB-pabA′-′lacZ CmrThis study
W168 rpoB18 (Rifr)BGSC
  • a Ptrp, Ppab, and PtrpP denote the trpEDCFBA, folate, and trpP operon promoters, respectively. A prime indicates truncation of the gene.

  • b BGSC, Bacillus subtilis Genetic Stock Center, Ohio State University, Columbus, Ohio.