TABLE 1.

Plasmids and gonococcal strains constructed and/or used in this study

Plasmid or strainGenotype and phenotypeSource or reference
Plasmids
    pHP45ΩSource for Ω (Spr Smr) cassette 32
    pNC40Source for cat (Cmr) cassette 46
    pUNCH173 tonB::Ω 3
    pUNCH1306 hemA::Ω 48
    pUNCH288Partial FA7168 pilQ1 in pCR2.1-TOPOThis study
    pUNCH290 pilQ1::Ω of p288This study
    pUNCH291 pilQ1::cat of p288This study
Strains
    FA1090Parent strain; wild type 15
    FA1090 H+HpuAB phase on variant of FA1090 7
    FA7167ΔhpuA hpuB::cat; Cmr Hb 6
    FA7168Cms Hb+ revertant of FA7167; pilQ1 6
    FA7169ΔhpuA hpuB+ derivative of FA7167; Cms Hb 6
    FA7186 hmbR::Ω transformant of FA7169; Spr Hb 6
    FA7186H1 hgbX2; Spr Hb+ revertant of FA7186 6
    FA7186H12 hgbX3; Spr Hb+ revertant of FA7186This study
    FA7186H15 hgbX4; Spr Hb+ revertant of FA7186This study
    FA7186H19Spr Hb+ revertant of FA7186; pilQ1This study
    FA7186H20 hgbX6; Spr Hb+ revertant of FA7186This study
    FA7294FA7186H19 pilQ1 transformant of FA7167; Cms Sps Hb+This study
    FA7311 pilQ::Ω transformant of FA1090 H+; Spr Hb+This study
    FA7312 pilQ1::Ω transformant of FA7294; Spr HbThis study
    FA7317FA7168 pilQ1 transformant of FA7167; Cms Sps Hb+This study
    FA7318FA7186H19 pilQ1 transformant of FA7167; Cms Sps Hb+This study
    FA7319 pilQ::Ω transformant of FA7167; Spr HbThis study
    FA7320 pilQ1::Ω transformant of FA7317; Spr HbThis study
    FA7321 tonB::Ω transformant of FA7317; Spr Hb+This study
    FA7322 hemA::Ω transformant of FA7317; Spr Hb+This study
    RM11.2recA6Defined P+ variant of FA1090 with IPTG-regulated recA 25
    FA7332 (RM7167)ΔhpuA hpuB::cat transformant of RM11.2recA6; HbThis study
    FA7333 (RM7168)FA7168 pilQ1 transformant of FA7332; Hb+This study
    FA7334 pilT::erm transformant of RM11.2recA626
    FA7335 pilQ1 pilT::erm transformant of RM11.2recA6This study
    FA7336ΔpilE of RM11.2recA6This study
    FA7337ΔpilE transformant of FA7332This study
    FA7338ΔpilE transformant of FA7333This study
    FA7339 pilQ1 transformant of RM11.2recA6This study
    FA7340 pilT::erm transformant of FA7333This study
    FA7341 pilT::erm transformant of FA1090This study
    FA7342 pilT::erm transformant t of FA7168This study
    FA7343 pilT::erm transformant of FA7186H19This study
    FA7344 pilT::erm transformant of FA7317This study
    FA7345 pilT::erm transformant of FA7318This study
    FA7348 pilT::tet transformant of FA1090This study
    FA7349 pilT::tet transformant of FA7167This study
    FA7350 pilT::tet transformant of FA7186H19This study
  • a Spr, SPT resistance; Smr, streptomycin (100 μg/ml) resistance; Cmr, CHL resistance.