TABLE 4.

The pilQ point mutation does not increase Hb binding to whole cells

StrainPhenotypeGrowth condition% Total cpm loadeda ± SD
FA1090 (HpuAB phase on)Hb+Iron replete0.24 ± 0.03
Iron limited1.51 ± 0.36
FA7167 (ΔhpuA hpuB::cat)HbIron replete0.07 ± 0.02
Iron limited0.05 ± 0.03
FA7168 (ΔhpuA hpuB::cat pilQ1)Hb+Iron replete0.08 ± 0.04
Iron limited0.08 ± 0.05
  • a 125I-labeled anti-human Hb antibody bound to gonococcal bound Hb is expressed as a percentage of the total counts per minute added to each well. Data are averages of two experiments, each done in quadruplicate.