TABLE 1.

Strains, plasmids, and oligonucleotides used in this work.

Strain, plasmid, or oligonucleotideGenotype, characteristic, or sequenceReference
E. coli strain
    DH5αsupE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1Lab stock
B. subtilis strains
    CU1065W168 attSPβ trpC2Lab stock
    ZB307AW168 SPβc2Δ2::Tn917::pBSK10Δ660
    HB7007CU1065, but sigX::spc20
    HB7013CU1065, but rsiX::pVA29 (MLSr)20
    HB0042CU1065, sigW::kan6
    HB0010CU1065, rsiW::kan6
    HB0030CU1065, sigX::spc, sigW::MLS8
    HB4035CU1065, sigD::kanLab stock
    HB4229HB1000, flgM::mini-Tn10 (Spcr)15
    HB0059CU1065, flgM::mini-Tn10 (Spcr)This work
    SDB01Marburg 160, but psd1::neo33
    SDB02Marburg 160, but pssA10::spc33
    HB0036ZB307A transduced with SPβ(PdltA1-cat-lacZ)This work
    HB0037ZB307A transduced with SPβ(PdltA2-cat-lacZ)This work
    HB0038CU1065, but dltA::pMUTINThis work
    HB0048CU1065, dltA::spcThis work
    HB0094CU1065, but dltA::pMUTIN and pssA::spcThis work
    HB0095CU1065, but dltA::spc and psd::neoThis work
    HB0089ZB307A transduced with SPβ(PywnJ-cat-lacZ)This work
    HB4514ZB307A transduced with SPβ(PpbpX-cat-lacZ)This work
    HB4509ZB307A transduced with SPβ(PpssA2-cat-lacZ)This work
    HB4533ZB307A transduced with SPβ(PpssA1-cat-lacZ)This work
    HB4519CU1065, but psd::neoThis work
    HB4520CU1065, but pssA::spcThis work
Plasmids
    pJM122Vector for integration of reporter fusions into SPβ51
    pGEM-cat-3Zf(+)Cloning vector for gene knockout59
    pJM114Kanamycin resistance cassette vector42
    pMUTIN4Cloning vector for integration, allows lacZ fusion at locus56
    pKF59Spectinomycin resistance cassette vector5
    pMC54PdltA1-cat-lacZ in pJPM122This work
    pMC55PdltA2-cat-lacZ in pJPM122This work
    pMC57dltA cloned in SacI-PstI of pGEM-cat-3Zf(+)This work
    pMC58spc cassette cloned in pMC57 (dltA::spc)This work
    pMC59an internal fragment of dltA cloned in EcoRI-BamHI of pMUTIN4This work
    pMC94PywnJ-cat-lacZ in pJPM122This work
    pJQ20PpbpX-cat-lacZ in pJPM122This work
    pJQ22PpssA2-cat-lacZ in pJPM122This work
    pJQ23PpssA1-cat-lacZ in pJPM122This work
Oligonucleotides
    368 dltA-r1235′-TCGCGGATCCATAATTCCTGATACGTGA-3′This work
    371 dltA-in-f5′-CCCGGAATTCCGTCCGAACGGATTG-3′This work
    372 dltA-in-r5′-CCCGGATCCATCAGGCACATTTGC-3′This work
    373 dltA-f15′-GCCCAAGCTTATTTGTTTGGTTCATCTTCC-3′This work
    374 dltA-f35′-GCCCAAGCTTTATTATGAATCAGCTCGAAAC-3′This work
    427 dltA-f5′-AAGCGAGCTCCTTGCAGGTATAAAGATT-3′This work
    428 dltA-r5′-AAAACTGCAGCCAAGCAGTATAAAGAAT-3′This work
    413 pbpX-f5′-AATGATAAGCTTGGCTGAGTGAAAAACTCAGC-3′This work
    414 pbpX-r5′-CAGGGATCCTCTTTTATTTAGTTTTCTCCG-3′This work
    422 pssA-f15′-TTTGGGAAGCTTATCTCTGGATCAGCCAG-3′This work
    407 pssA-f25′-TTTGGGAAGCTTCTATGTTATCATGCTTATTGG-3′This work
    408 pssA-r5′-TGAGGATCCAGCAATCCGCAAATGAAG-3′This work
    537 psd-in-f5′-GGATTTTGCGAGTCGAAA-3′This work
    538 psd-in-r5′-CTCCAATTCAGTACGGGT-3′This work
    571 ywnJ-f5′-CGCAAGCTTACCCAAGAAACAGAAGAA-3′This work
    572 ywnJ-r5′-CCCGGATCCACAGACAGAAAGCAGGAT-3′This work