TABLE 1.

Primers used in this study

PrimerNucleotide sequenceaLocationbComments
F-BC-1GGAAGTGTTTTCACAAATGTGTCGSignal peptide, cipBc (scaA)Probe for library screening
R-BC-1CCAATCAAACACCATAGTACCGTCCoh-1, scaAProbe for library screening
F-BC-AN-1GCCTGATTATGGAACATTGTTGCAGGGCoh-11, scaAProbe for library screening
R-BC-AN-1CTGCTGGGAACTCGGAAATCATTCCDoc, scaAProbe for library screening
Coh-FAGACCATGGGTTCAGGAGTAGTAGCAACCoh5, scaAExpression, NcoI, His tag
Coh-RAGTCTCGAGTCCGTTTATTGAAGAAGCCTGCoh5, scaAExpression, XhoI, His tag
PBC-Coh11-NCTGCCATGGGATCTGTATTGACAGCTCoh11, scaAExpression, NcoI, His tag
PBC-Coh11-CTACCTCGAGTGTGCCTTTTGGATAGATCoh11, scaAExpression, XhoI, His tag
F-EX-BCcohB3GGGCCATGGGGAAAAGTTCACCAGGAAATAAAATGCoh3, scaBExpression, NcoI, His tag
R-EX-BCcohB3CCCCTCGAGATTAGTTACAGTAATGCTTCCATCCoh3, scaBExpression, XhoI, His tag
F-9dxyn-docGH48ATAGGTACCTCCATTTGTTAAATTAAAAGGTGACDoc, cel48AExpression, KpnI, fused to Xyn T6
R-9dxyn-docGH48CCCGGATCCAAACATTACGACCTCTGCTGATTTDoc, cel48AExpression, BamHI, fused to Xyn T6
F-9dxyn-docCipBcATAGGTACCTCCAAAAGGCACAGCTACAGTATTADoc, scaAExpression, KpnI, fused to Xyn T6
R-9dxyn-docCipBcGGGGGATCCTTTTTGTTCTGCTGGGAACTCGGADoc, scaAExpression, BamHI, fused to Xyn T6
  • a Residues shown in italics were added to the 5′ ends to generate the designated restriction enzyme cleavage sites (underlined [see Comments column]).

  • b Abbreviations: Doc, dockerin domain; Coh, cohesin domain.