TABLE 3.

Protection of AMO by allylthiourea but not by AMO substrates or anaerobic conditionsa

ProtectantMean AMO activity with DPI only (% of control) ± SDMean AMO activity with DPI and protectant (% of control) ± SD
Allylthiorrea (1 mM)47.6 ± 5.790.4 ± 6.8
Methane (3 ml)27.0 ± 8.422.1 ± 7.4
Ethane (3 ml)27.2 ± 6.223.2 ± 5.1
Phenol (1 mM)23.3 ± 3.926.5 ± 4.9
Toluene (1 mM)32.9 ± 526.7 ± 6.6
Anaerobic36.8 ± 7.119.2 ± 9.3
Hydrazineb (500 μM)77.4 ± 5.943.3 ± 4.8
  • a For each protectant, three samples of N. europaea cells were prepared: one with 200 μM DPI, one with 200 μM DPI and the indicated protectant, and a control with no DPI and no protectant. These samples were incubated at room temperature for 5 min, washed four times with 50 mM phosphate (pH 8), and assayed for AMO activity by measuring the rate of ethylene oxidation as described in the text. Activities are given relative to the activity of the control sample and represent the mean of three measurements.

  • b These samples contained 100 μM DPI rather than 200 μM as in all the other samples.