TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Sourcea or reference
B. subtilis
    168trpC21
    168-PRtrpC2 phoRΔBalI::Tcr30
    ML6trpC2 sigBΔHindIII-EcoRV::Cmr18
    168-PR-SBtrpC2 phoRΔBalI::Tcr sigBΔHindIII-EcoRV::CmrML6→168-PR
    SWV119trpC2 pheA1 abrB::Tcr44
    168-ABtrpC2 abrB::TcrSWV119→168
    SWV215trpC2 pheA1 spo0A::Kmr44
    168-0AtrpC2 spo0A::KmrSWV215→168
    BHItrpC2 pheA1 spo0HΔHindIII-EcoRI::Cmr11
    168-SHtrpC2 spo0HΔHindIII-EcoRI::CmrBHI→168
    650trpC2 ilvB2 leuB16 spoIIAABC::CmrJ. Errington
    168-SFtrpC2 spoIIAABC::Cmr650→168
    901trpC2 spoIIGA::Kmr43
    168-SEtrpC2 spoIIGA::Kmr901→168
Plasmids
    pBluescript II KS(+)Phagemid cloning vector Apr (2.961 kb)Stratagene
    pNHPpBluescript II KS(+) containing a 621-bp insert of phoP Apr (3.558 kb)This study
    pNHRpBluescript II KS(+) containing a 1,530-bp insert of phoR Apr (4.467 kb)This study
    pPEpBluescript II KS(+) containing a 451-bp insert of phoP Apr (3.4 kb)This study
    pET2816164-bp XbaI-BlpI fragment of pET16b replaced with the 255-bp XbaI-BlpI fragment of pET28a(+) (Novagen) Apr (5.802 kb)T. Msadek
    pET-PhoPpET2816 containing a 722-bp insert of phoP Apr (6.386 kb)This study
    pET-PhoR231pET2816 containing a 1,049-bp insert of phoR Apr (6.713 kb)This study
  • a Arrows indicate transformation from donor to recipient.