TABLE 2.

Expressed proteins prepared in this study

ProteinModular contentPlasmidTag
CohA5Cohesin 5 of CipBc (ScaA)pET28aC-terminal His tag
CohA11Cohesin 11 of CipBc (ScaA)pET28aC-terminal His tag
CohB3Cohesin 3 of ScaBpET28aC-terminal His tag
Xyn-DocAHybrid construct consisting of G. stearothermophilus Xyn T6 harboring the B. cellulosolvens ScaA dockerin at the C terminuspET9dN-terminal His tag
Xyn-DocGH48Hybrid construct consisting of Xyn T6 and the B. cellulosolvens Cel48A dockerin at the C terminuspET9dN-terminal His tag