TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsSource or reference
Bacillus subtilis strains
    168 trpC2 Laboratory stock
    BFS219168 clsA (formerly ywnE)::pMutin4P. Stragier
    BFS1244168 ywiE::pMutin4M.-F. Hullo
    BFS1245168 ywjE::pMutin4M.-F. Hullo
    SDB201168 ywjE1::spcThis study
    SDB202168 ywiE2::neoThis study
    SDB203BFS219 ywiE2::neoThis study
    SDB206SDB203 ywjE1::spcThis study
Escherichia coli SD9 cls-1 pssA1 34
Plasmids
    pBR322Tetr Ampr4
    pSK6pSC101 having a multiple-cloning site and PBAD of pBAD24, Ampr46
    pMMS1pSK6 harboring clsA under control of PBADThis study
    pMMS2pSK6 harboring ywjE under control of PBADThis study
    pMMS3pSK6 harboring ywiE under control of PBADThis study
    pDG1726 spc 16
    pBEST502 neo 21
    pJESPCpBR322 ywjE::spcThis study
    pIENEOpBR322 ywiE::neoThis study