TABLE 1.

Strains and plasmids used

Strain or plasmidDescriptionaSource and/or reference
B. amyloliquefaciens
    FZB42Producer of surfactin, fengycin, and bacillomycin DFZB Biotech GmbH Berlin (11)
    AK1ΔbmyA::EmrThis study
    AK2ΔfenA::CmrThis study
    AK3ΔbmyA::Emr ΔfenA::CmrThis study
    CH1ΔsrfA::EmrThis study
Plasmids
    pMX39Apr Emr TetrDerivative of pDB101 (3)
    pDG364 amyE; Apr CmrIntegrative vector (6)
    pGEM-TCloning vector; AprLaboratory stock
    pAK1pGEM-T carrying a 1.2-kb fragment from bmyA; AprThis study
    pAK2pGEM-T carrying bmyA::Emr; Apr EmrThis study
    pAK3pGEM-T carrying a 1.3 kb fragment from fenA; AprThis study
    pAK4pGEM-T carrying fenA::Cmr; Apr CmrThis study
    pCH1pGEM-T carrying a 2.3-kb fragment from srfA::Emr; AprThis study
  • a Apr, ampicillin resistance; Emr, erythromycin resistance; Tetr, tetracycline resistance.