TABLE 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidCharacteristicsReference
Bacterial strains
    E. coli DH5αMCRF mcrAΔ(mrr-hsd RMS-mcrBC) recA φ80dlacZ ΔM15Stratagene
    X. oryzae pv. oryzae
        T7174RSpontaneous mutant of T7174, Rfr, used as the wild-type strain in this study11, 34
        74ΔHrpXoTransposon insertion mutant of T7174R, hrpXo mutant, Rfr Kmr30
        74ΔHrcVTransposon insertion mutant of T7174R, hrcV mutant, Rfr Kmr13
        74ΔXpsETransposon insertion mutant of T7174R, xpsE mutant, Rfr KmrThis study
        74ΔXpsLTransposon insertion mutant of T7174R, xpsL mutant, Rfr KmrThis study
        74ΔXpsNTransposon insertion mutant of T7174R, xpsN mutant, Rfr KmrThis study
        74ΔCysP2Transposon insertion mutant of T7174R, cysP2 mutant, Rfr KmrThis study
    X. axonopodis pv. citri MAFF302104a
Plasmid
    pBluescript II SK+Phagemid, pUC derivative, AmrStratagene
    pUC119Plasmid, Amr33
    pBSGUSgus gene in pBluescript II KS(+)31
    pHM1Broad-host-range vector with pUC19 polylinker, Spr17
    pHMCysP2PIPPromoter region of cysP2 is inserted in pHM1This study
    pHMCysP2GUScysP2::gus fusion gene is inserted in pHM1This study
    pGLCysPCosmid clone from the genomic library of T7174R containing cysP2This study
    pUCCysP25-kb EcoRI-HindIII fragment of pGLCysP is inserted in pUC119This study
    pUCΔCysP2Transposon insertion in cysP2 of pUCCysP2This study
  • a MAFF, Collection of Ministry of Agriculture, Forestry, and Fisheries, Tsukuba, Japan.