TABLE 1.

Bacterial strains and plasmids used in this work

Strain or plasmidDescriptionSource or reference
Strains
    XL1BluerecA1 lac endA1 gyrA96 thi hsdR17 supE44 relA1 (F′ proAB lacIq lacZΔM15 Tn10)Stratagene
    TE2680F λ IN(rrnD-rrnE) ΔlacX74 rplS galK2 recD::Tn10d-tet trpDC700::putA13033::(Kanr Cmr lac)10
    JMH0317W3110 rpoS::Tn10J. Membrillo-Hernandez
    JA134ECL1 Lyx+25
    JA182JA134 Δ(tyrR-fnr-nac-trg)17 zdd-230::Tn914
    JA184JA134 himA::cat14
    JA185JA134 himD3::cat14
    JA188JA134 rpoS::Tn10This work
    BL21(DE3)E. coli B F ompT hsdS (rB mB) gal dcm λ(DE3)Pharmacia Biotech
    JA211JA134 yiaR::catΔulaR6
    JA216JA211 crp::catThis work
Plasmids
    pRS550/pRS551Apr Kmr; contains promoterless lacZYA28
    pET43c (+)Nus tag bla (Carbr) lacINovagen
    pET-UlaRulaR in pET43c (+)This work
    pBS-CRPcrp in pBluescriptThis work