TABLE 2.

β-Galactosidase activities of Y. pestis containing either the hmsHFRS::lacZ or hmsT::lacZ reportera

StrainMean β-galactosidase activity: (Miller units) of cells grown atb:
26°C in deferrated PMH237°C in deferrated PMH226°C in HIB37°C in HIB
NAc10 μM FeCl310 μM HeminNA10 μM FeCl310 μM HeminExponentialEarly stationaryLate stationaryExponentialEarly stationaryLate stationary
KIM6-2097 (hmsHFRS::lacZ)11,192 ± 1,6538,422 ± 1,7337,614 ± 1,61815,012 ± 2,86113,180 ± 2,53011,772 ± 3,0543,411 ± 4265,438 ± 7698,959 ± 2,0234,584 ± 5896,709 ± 4448,526 ± 1,127
KIM6-2096+ (hmsT::lacZ)7,071 ± 1,2816,686 ± 2,8405,873 ± 1,9859,198 ± 2,4377,933 ± 9937,410 ± 1,1562,325 ± 4782,724 ± 7874,256 ± 7636,056 ± 1,5847,499 ± 3,70812,844 ± 1,068
  • a Cells were harvested during exponential, early-stationary, and late-stationary phases of growth (HIB cultures) or only during exponential-phase growth (PMH2 cultures).

  • b Enzyme activities are expressed in Miller units (33). The values shown (mean±standard deviations) are the average of two or more individual reactions from three or more independent cultures.

  • c NA, no additions