TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    E. coli
        NovaBlueRecipient for cloning experimentsNovagen
        BL21(DE3)/pLysSUsed for overproduction of proteins from pET-based vectorsNovagen
    Salmonella
        JR501Used for converting E. coli plasmids to Salmonella compatibility28
        SJW1103Wild type33
        SJW170fliR null33
        MKM50flhB null strain6
        MKM65fliR null strainThis study
        MKM58fliR flhB double-null strainThis study
Plasmids
    pET19bN-terminally His-tagged T7 expression vectorNovagen
    pET19bFHN-terminally His-FLAG-tagged T7 expression vector4
    pTrc99A-FF4Modified pTrc expression vector27
    pMM26pTrc99A-FF4/FlhB22
    pRMK3000pET19b/N-His-FliRThis study
    pGFBN269Apet19b/N-His-FlhBN269A6
    pJSV003pET19bFH/N-His-FLAG-FliR-FlhBThis study
    pJSV004pTrc99A-FF4/FliR-FlhBThis study
    pJSV013pET19bFH/N-His-FLAG-FliR-FlhBN269AThis study
    pJSV014pTrc99A-FF4/FliR-FlhBN269AThis study
    pJSV024pTrc99A-FF4/FliR + FlhBThis study