TABLE 5.

MinD-independent membrane localization of, and division inhibition by, a ZipA′-GFP-MinC fusion protein

Phage or plasmidFusion proteinIPTGa (μM)Relative level of fusion proteinbLoccLength (μm)ne
AvgRanged
None756.01.8-31.4131
λCH178ZipA′-GFP751.5M5.71.9-41.9113
λDR121GFP-MinC750.7C5.11.8-17.7122
λDR121GFP-MinC2501.1C5.61.6-59.0138
pDR121GFP-MinC758.6C9.61.7-65.0131
λPC100ZipA′-GFP-MinC751.0M20.73.1-114.4133
λLL49ZipA′-GFP-DMinC751.8M6.22.1-65.0134
  • a Cells of strain LL1 (ΔminCDE lon), harboring the indicated phage or plasmid, were grown at 37°C to an OD600 of 0.2 to 0.3 in minimal medium supplemented with IPTG as indicated.

  • b Protein levels were measured by quantitative Western analyses, using anti-GFP antibodies. Values are normalized to that of the ZipA′-GFP-MinC fusion in LL1 (λPC100).

  • c The localization (Loc) of fusion proteins, and the average cell lengths, were determined by microscopy. M, concentrated along the membrane; C, cytoplasmic.

  • d Note that cultures of LL1 normally contain a small fraction of filaments due to the lon mutation in this strain.

  • e Number of cells measured.