TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource and/or reference
E. coli BL21(DE3)B F dcm ompT hsdS (rB mB) gal λ (DE3)Stratagene
S. aureus strains
    MN8tstH1
    RN42208325-4 rK mK+16
    MN3050RN4220(pJMY10) tstH+37
    MN4011RN4220 srrBΩpJMY2 Emr (pJMY11) tstH+ srrAB+37
    DU58758325-4 Δspa::Tcr, TcrT. Foster (20)
    DU5875(pJMY11)DU5875(pJMY11), Tcr CmrThis study
    RN63908325-4R. Novick (23)
    RN6390(pJMY10)8325-4(pJMY10), CmrThis study
    RN6390(pJMY11)8325-4(pJMY11), CmrThis study
Plasmids
    pJMY10805-bp Cmr cassette from pIJ002 cloned into the PstI and ScaI sites of pCE107, tstH, 5.4 kb, Cmr37
    pJMY112,712-bp srrAB operon cloned into the XmaI site of pJMY10, tstH srrAB, 8.2 kb, Cmr37
    pJMY21402-bp portion of srrB (SrrB*) cloned into NheI-BamHI sites of pET28bThis study
    pJMY22726-bp full-length srrA cloned into NheI-BamHI sites of pET28bThis study