TABLE 2.

Localization of DicB-GFP-DMinC in λJE64 lysogens and of GFP-DMinC in λJE65 lysogens

StrainRelevant genotypeSpecial conditionaLocationb
λJE64 (DicB-GFP-DMinC)λJE65 (GFP-DMinC/MinD)
DR109ΔminCDERR
DR109/pBAD33ΔminCDE/PBAD::ARARR
DR109/pJE80ΔminCDE/PBAD::sfiAARACcMc
JE15/pDB280ΔminCDE ftsA0recA/repA(Ts) ftsA30°CRR
42°CRcRc
CH28ΔminCDE ΔzapARR
JE25/pCH32ΔminCDE ΔzipA recA/repA(Ts) zipA ftsZ30°CRR
42°CCcRc
JE23ΔminCDERR
JE35ΔminCDE ftsA*RR
JE33ΔminCDE ΔzipA ftsA*CR
JE23/pZAQΔminCDE/ftsQAZRR
JE24/pZAQΔminCDE ΔzipA/ftsQAZCR
  • a Unless indicated otherwise, cells were grown at 37°C in LB-AMP medium supplemented with 50 μM IPTG to an OD600 of 0.2 to 0.5. Where indicated, 0.1% arabinose (ARA) was included in the medium.

  • b Over 100 cells were examined in each case. R, concentrated in one or more rings in over 70% of the population; M, concentrated along the cell periphery, less than 1% of cells showed obvious rings; C, cytoplasmic, less than 1% of cells showed obvious rings.

  • c Cells had a Sep division phenotype, ranging in length from 15 to 60 μm. Cells were Min in all other cases.