TABLE 1.

β-Galactosidase assays measuring rate of transcription from qsrAB and comM promoters in the presence of CSP-1 and/or BIP-1

StrainPromoterPheromoneActivity (Miller units)a
EK4144qsrAB (p1 and p2)bNone80.4 ± 2.19
CSP-1176.7 ± 1.7
BIP-1120 ± 4.33
CSP-1 + BIP-1217 ± 2.14
2 × CSP-1181.3 ± 2.5
OE 4144qsrAB (p1)cNone0.58 ± 0.07
CSP-113.3 ± 0.99
BIP-14.89 ± 0.61
CSP-1 + BIP-115.25 ± 1.18
OE 4145qsrAB (p1)dNone0.41 ± 0.06
CSP-110.12 ± 1.16
BIP-110.60 ± 1.80
CSP-1 + BIP-114.84 ± 1.87
OE 4146qsrAB (p1)dNone0.46 ± 0.02
CSP-111.60 ± 0.98
BIP-11.01 ± 0.24
CSP-1 + BIP-111.63 ± 1.31
OE 4147qsrAB (p1)dNone0.82 ± 0.16
CSP-17.55 ± 0.38
BIP-13.24 ± 0.80
CSP-1 + BIP-110.01 ± 1.52
OE 4148qsrAB (p1)dNone0.79 ± 0.23
CSP-18.11 ± 0.65
BIP-13.19 ± 0.50
CSP-1 + BIP-19.25 ± 0.43
OE 4149qsrAB (p1)dNone0.20 ± 0.1
CSP-10.30 ± 0.06
BIP-10.34 ± 0.03
CSP-1 + BIP-10.34 ± 0.03
EK4252comMNone2.42 ± 0.12
CSP-117.64 ± 0.12
BIP-12.18 ± 0.80
CSP-1 + BIP-119.48 ± 0.58
2 × CSP-115.95 ± 0.58
  • a β-Galactosidase activities are given in Miller units, and are the means ± standard errors of triplicate samples.

  • b Activity is the sum of the activities of the competence-inducible promoter (p1) and the housekeeping promoter (p2).

  • c Activity is the activity of only the competence-inducible promoter (p1).

  • d Activity is that of the p1 promoter containing different mutations.