TABLE 2.

β-Galactosidase assays demonstrating specificity of the CSP-1 and BIP-1 pheromones in stimulating the rate of transcription of the qsrAB operon in the EK4144 straina

Pheromone(s)Pheromone sequenceActivity (Miller units)b
None50.5
CSP-1N-EMRLSKFFRDFILQRKK-C89.8
2 × CSP-187.9
CSP-2N-EMRISRIILDFLFLRKK-C52.1
CSP-3165N-SQKGVYASQRSFVPSWFRKIFRN-C51.8
BIP-1N-GWWEELLHETILSKFKITKALELPIQL-C70.7
BIP-2N-GLWEDLLYNINRYAHYIT-C50.5
CSP-1 + BIP-1104.8
  • a The experiment was repeated several times with similar results.

  • b Sum of the activities of the competence-inducible promoter (p1) and the housekeeping promoter (p2).