Plasmid^{a} | Characteristics | PFO titer (log_{2})^{b} |
---|---|---|

pJIR751 | Shuttle vector, negative control | <1.0 |

pJIR1972 | pfoR^{+} pfoA^{+}, positive control | 5.9 ± 0.5 |

pJIR2373 | Cloning vector | 1.4 ± 0.1 |

pJIR2422 | Wild-type VirR boxes upstream of pfoA | 5.8 ± 0.5 |

pJIR2447 | Insertion of 5 bp between VirR boxes | 1.3 ± 0.03 |

pJIR2448 | Insertion of 10 bp between VirR boxes | 2.5 ± 0.1 |

pJIR2481 | Deletion of 5 bp between VirR boxes | 1.4 ± 0.1 |

pJIR2632 | Insertion of 5 bp between VirR box 2 and −35 box | 1.1 ± 0.4 |

pJIR2620 | Insertion of 10 bp between VirR box 2 and −35 box | 1.3 ± 0.4 |

pJIR2479 | VirR boxes from upstream of CPE0845 | 3.7 ± 0.3 |

pJIR2483 | VirR boxes from upstream of CPE0846 | 7.6 ± 0.3 |

pJIR2480 | VirR boxes from upstream of CPE0920 | 4.4 ± 0.1 |

pJIR2489 | VirR boxes from upstream of hyp7 | 6.7 ± 0.2 |

↵

*a*All plasmids were analyzed in strain JIR4228.↵

*b*PFO titer refers to the mean perfringolysin O titer (± standard deviation) obtained from duplicate assays using supernatants from three independent cultures of each strain. Note that these titers are reported on a logarithmic, not arithmetic, scale.