TABLE 4.

Expression of DAS-rocG-lacZ fusionsa

StrainRelevant genotypePromoter region within the fusionβ-Galactosidase activity (U)
Medium with glucose plus:Medium without glucose but with:
NH4ClGlutamateProlineOrnithineProlineOrnithine
BB1512Wild typerocGp+0.120.7611.4b11.935.1233.9
BB1521ccpArocGp+1.61.419.6187.850.1160.8
BB2131rocR(T120I)rocGp+3.613.514.69.6138.7228.8
BB2139ccpA rocR(T120I)rocGp+NGc247.7268.6250.0252.1279.2
BB2063Wild typerocGp10.161.1275.8251.093.9336.4
BB2064ccpArocGp11.21.011.2242.0
BB2132rocR(T120I)rocGp156.5166.0245.1200.0
  • a Derivatives of strains BB1512 and BB2063 carrying DAS-rocG-lacZ fusions at the ectopic amyE locus (with the rocG DAS sequence positioned upstream of the fusion promoter) were grown in minimal medium with the indicated carbon and nitrogen sources and assayed for β-galactosidase activity.

  • b Low activity of the rocG-lacZ fusion (1 U) was detected during early exponential growth.

  • c NG, no growth under these conditions.