TABLE 1.

Phenotypes displayed by wild-type isolates and isogenic flagellar protein export and filament mutants

Background strainRelevant characteristicFlaA synthesisFilament assemblyMotilitySecretionAdherenceaInvasionbI/Ac
F38011Wild type++++100 ± 5100 ± 205.3
flhB 47 ± 90.24 ± 0.040.03
flgB 40 ± 60.46 ± 0.10.06
flgC 73 ± 22.1 ± 0.50.13
flgE2 13 ± 20.26 ± 0.10.11
fliD ++++59 ± 844 ± 44.0
ciaB +++223 ± 151.9 ± 0.70.02
81116Wild type++++100 ± 9100 ± 272.7
flaA flaB 191 ± 2710 ± 30.14
flaA Truncated+145 ± 1970 ± 71.3
flaA flaB/pMEK3502 (flaA+)++++127 ± 1829 ± 10.63
flaA flaB pMEK3503 (flaB +)Truncated+255 ± 2743 ± 70.46
  • a As a percentage of the wild-type value, which was 9.4 × 105 ± 0.5 × 105.

  • b As a percentage of the wild-type value, which was 5.0 × 104 ± 1 × 104.

  • c I/A, [(number of internalized bacteria)/(number of adherent bacteria)] × 100.