TABLE 2.

GlutDH activity in minimal mediuma

Carbon sourceNitrogen sourceGlutDH activity (U)
SMY wild typeBB1179 ccpABB1597 ccpA rocR(T1201)BB1744 rocGp1BB1792 rocGp1 rocR(T120I)
GlucoseAmmonium≤45≤45NGb≤451,255
Glutamate≤45≤451,052≤451,005
Ornithine74681791677666
OrnithineOrnithine7691,113NDc1,147747
GlucoseProline766288363967
ProlineProline246177ND268ND
  • a Cells were grown in minimal medium with the indicated carbon and nitrogen sources and assayed for GlutDH activity (7).

  • b NG, no growth under these conditions.

  • c ND, not determined.