TABLE 1.

Cellular contents of chemotaxis components

ComponentContent (no. of molecules/standard cell vol)a in strain:
RP437OW1RP2867 (ΔcheR cheB)
Rich mediumMinimal mediumRich mediumMinimal mediumRich mediumMinimal medium
Receptors (total)b15,000 ± 1,70026,000 ± 1,8003,600 ± 17041,000 ± 1,400
    Tsr + Tar14,000 ± 1,70024,000 ± 1,8003,300 ± 17037,000 ± 1,3009,200 ± 600c12,000 ± 660c
    Trg440 ± 70910 ± 20150 ± 201,800 ± 200
CheA (total)6,700 ± 1,1007,700 ± 4401,100 ± 13010,000 ± 4605,100 ± 2905,000 ± 530
    CheAL4,500 ± 9405,100 ± 380810 ± 1106,700 ± 3403,200 ± 1903,300 ± 450
    CheAS2,200 ± 5202,600 ± 230340 ± 903,600 ± 3101,900 ± 2201,700 ± 280
CheW6,700 ± 8907,200 ± 8302,000 ± 20016,000 ± 1,2005,300 ± 1505,500 ± 510
CheY8,200 ± 3106,300 ± 701,400 ± 9014,000 ± 1,400
CheZ3,200 ± 902,700 ± 80560 ± 905,700 ± 130
CheB240 ± 10270 ± 1050 ± 10440 ± 20
CheR140 ± 10160 ± 1040 ± 10320 ± 10
  • a Means ± standard deviations for three independent cultures analyzed four to six times, except for Tsr and Tar in RP2867 (two cultures).

  • b Sum of the experimental measurements for Tsr + Tar and Trg and an estimate for Tap and Aer. See text for details.

  • c Sum of separate values for Tsr and Tar. Tsr rich, 5,200 ± 520; minimal, 6,100 ± 510. Tar rich, 4,000 ± 290; minimal, 5,800 ± 410.