TABLE 1.

Bacterial strains and plasmids used throughout this study

Strain or plasmidCommentsaSource or reference
Strains
    S. intermedius 15.3T.2Tcr donor strainThis study
    B. subtilis BS34ATcr B subtilis::Tn91611
    E. faecalis JH2-2Rifr recipient strain14
    E. coli pAT451Tcr E. coli::pAT451b1
    S. sobrinus NCTC 12279 (type strain)Rifr recipient strainHealth Protection Agencyc
    S. sanguinis NCTC 7863 (type strain)Rifr recipient strainHealth Protection Agency
Plasmids
    pAM120pUC18::Tn9165
    pAT451pUC18::tet(S)1
  • a Tcr; tetracycline resistant, Rifr; rifampin resistant.

  • b pUC18 carrying a 4.5-kb ClaI fragment of pIP811 with the tet(S) gene.

  • c www.hpa.org.uk.