TABLE 2.

Oligonucleotide primers used in this study

Primer namePrimer sequence or referenceExpected PCR product
1-2615aorf24 to orf14 of Tn916
RT15′-CTCTATCCTACAGCGACAGC-3′PCR product containing orf13, orf12, tet(M), and orf6
RT45′-TCTTTGCGTCTGGCTCTGTA-3′
RT65′-TATGATTTTAGAGCCCTTTGG-3′PCR product containing orf6 and orf9
RT115′-ACAGAGCCAGACGCAAAGAC-3′
RT75′-CTTGTATGCTGGGGTGTTGC-3′PCR product containing orf9 and orf7
RT145′-TAATTCTTCCGCTCGTCGTC-3′
RT135′-AAAGAAAGGGGGTGAAC-3′PCR product containing orf7 and orf8
RT155′-GGTTAATCGCTTCTGTATCG-3′
RT125′-ATTTCACGTTTCTTGTCTGG-3′Primer reading upstream from within orf7
RT185′-AAGTATGGTCGTTGATGAAG-3′Primer reading downstream from within orf8
SRV7667-bp product of the tet(S) gene
SFW7
intxis16xis and int fragment of Tn916
intxis26
REO14Joint of the circular form of Tn916
LEO14
SFW(2)5′-GTGTCCAGGAGTATCTAC-3′Sequencing between RT1 and RT4
RT1(2)5′-CTGTCAATTAGATAGCGGG-3′Sequencing between RT1 and RT4
RT4rev5′-GATTTGAATTAAAGTGTAAAGGAGG-3′Primer reading downstream from within orf6
RT7rev5′-ATGTATAGAGTGGTCTACTATGCG-3′Primer reading upstream from within orf9
  • a Thirteen pairs of sequences.