TABLE 2.

Southern hybridization with right-end DNA probes

CulturecRepliconbProbea
12345
Sh-2-82 (p7)Chromosome++++++++++
lp18(+)(+)
Sh-2-82 (p166)ChromosomeND++++++++
lp26(+)
lp28(+)
Sh-2-82 (p320)Chromosome++++++++++
Linear plasmids
JD1 (p16)Chromosome++ND
lp26++++++
297 (p6)Chromosome++dNDNDND++
lp29(+)NDNDNDND
21305 (p < 20)Chromosome++ND
lp25++++++
lp27+
22921 (p8)Chromosome++ND
lp24++++++
lp27+
28534 (p4)Chromosome++++ND++++
lp26+(+)++
29968 (p < 20)Chromosome++++ND++++
lp23+
lp26(+)++
30757 (p5)Chromosome+NDND
lp24++
  • a Probes are described in Table 1 and Fig. 1. Table symbols are as follows: −, no hybridization; +, moderate hybridization; ++, strong hybridization; ND, not done. Parentheses in the table data indicate very weak hybridization that most likely represents cross-hybridization to nonorthologous, paralogous sequences.

  • b Hybridization with the chromosome includes the demonstration that this hybridization is with the rightmost BssHII and SgrAI restriction fragments. The numbers in the linear plasmid “lp” plasmid names indicate only the apparent size in kilobase pairs and do not indicate any specific relationship to plasmids in other strains with similar sizes or names.

  • c The number of culture passages since the original isolation of the strain is given in parentheses. “p < 20” indicates that cultures were described by the supplier as “low passage.”

  • d A DNA probe that overlaps probe 1 but is not identical to it was used in the case of strain 297 (see text).