TABLE 2.

Glutamine synthetase activities in E. coli DH5α strains that express the FlgR AAA+ domain or the DctD AAA+ domain

PlasmidProtein expressedGlutamine synthetase activity (nmol of γ-glutamyl hydroxymate/min/A650 unit)a
−IPTG+IPTG
NoneNone5.46 ± 1.226.38 ± 2.00
pPBHP80FlgR AAA+ domain17.4 ± 5.4215.4 ± 3.14
pHX182DctD AAA+ domain16.5 ± 5.079.62 ± 3.40
  • a Values represent the averages of at least six replicates ± standard deviations. −IPTG, without IPTG induction; +IPTG, with IPTG induction.