TABLE 4.

Transformation mapping of the cmp locusa

Transformation recipientTransformation donorTransformant phenotypeFrequency (%)
MH5857 (cmp)
    Cross 1MH5881 (tatCY::Spcr ydhQ::Ermr)Spcr Erms cmp41
Spcr Erms38
Spcr Ermr14
Spcr Ermr cmp7
    Cross 2MH5874 (tatCY:Spcr rrnE-23s:Knr)Spcr Kns59
Spcr Knr21
Spcr cmp Kns15
Spcr cmp Knr5
  • a The frequency of each transformant obtained is shown. In cross 1, three-factor cross transformation using MH5881 (ΔydhQ ΔtatCY) as the donor strain and MH5857 (cmp) as the recipient strain was selected on Spcr and screened for Ermr and the cmp phenotype. In cross 2, three-factor cross transformation using MH5874 (ΔtatCY rrnE-23s::Tn10-2) as the donor strain and MH5857 (cmp) was selected on Spcr and screened for Neor and for the cmp phenotype.