TABLE 2.

Sequence analysis of psl gene cluster

Gene no.Gene nameCOG/pfam identification and descriptiona
PA2231 pslA COG2148/pfam02397, sugar transferases
orf477 COG1086, predicted nucleoside-diphosphate sugar epimerases; pfam02719, polysaccharide biosynthesis protein
PA2232 pslB COG0836/pfam00483, mannose-1-phosphate guanylyltranferase
orf488 COG0662/pfam01050, mannose-6-phosphate isomerase
PA2233 pslC COG1216, predicted glycosyltransferases
orf303 pfam00535, glycos_transf_2, glycosyl transferase; COG1215, glycosyltransferases, probably involved in cell wall biogenesis; COG0463, WcaA, glycosyltransferases involved in cell wall biogenesis
PA2234 pslD COG1596, Wza, periplasmic protein involved in polysaccharide export; pfam02563, polysaccharide biosynthesis/export protein
PA2235 pslE COG3206, GumC, uncharacterized protein involved in exopolysaccharide biosynthesis; pfam02706, Wzz, chain length determinant protein
PA2236 pslF COG0438/pfam00534, RfaG, glycosyltransferase; PF00534, glycosyltransferase, group 1 family protein
PA2237 pslG COG3664, XynB, beta-xylosidase; pfam01229, glyco_ hydro_39, glycosyl hydrolases family 39; PF00150, cellulase (glycosylhydrolase family 5)
PA2238 pslH COG0438/pfam00534, RfaG, glycosyltransferase; PF00534, glycosyltransferase, group 1 family protein
PA2239 pslI COG0438/pfam00534, RfaG, glycosyltransferase; PF00534, glycosyltransferase, group 1 family protein mannosyltransferase, WbkA (Brucella melitensis)
PA2240 pslJ PF04932, O-antigen polymerase (weak)
PA2241 pslK COG0728/pfam03023, MviN, uncharacterized membrane protein, putative virulence factor; PF01943, polysaccharide biosynthesis protein (weak)
  • a COG, clusters of orthologous groups.