TABLE 3

Cofactor specificities of purified AdhE proteins

Source of AdhEDescriptionALDH activityaADH activity
NADHNADPHNADHNADPH
C. thermocellum LL1004Wild type18.02 ± 2.45b2.03 ± 0.4642.23 ± 3.131.96 ± 1.25
C. thermocellum LL346Ethanol tolerant13.50 ± 3.540.43 ± 0.424.02 ± 1.480.03 ± 0.34
C. thermocellum LL350Moderate ethanol production31.50 ± 5.805.76 ± 0.1642.67 ±7.4642.30 ± 4.28
T. saccharolyticum LL1025Wild type10.63 ± 1.154.46 ± 1.6317.43 ± 2.452.43 ± 2.43
T. saccharolyticum LL1049High ethanol production1.63 ± 0.3011.13 ± 1.350.66 ± 1.9712.70 ± 2.18
T. saccharolyticum LL1040High ethanol production0.07 ± 0.555.03 ± 2.050.01 ± 1.3412.96 ± 4.54
  • a Activity is expressed in units per milligram of protein (see Materials and Methods).

  • b Error represents 1 standard deviation (n = 4 to 8).