TABLE 1.

Lectins binding Y. pseudotuberculosis biofilm on C. elegans

FITC-linked lectinaMonosaccharide binding specificitybLectin binding buffercLectin binding detected by fluorescence
BPAGalNAc1
ConAα-Man > α-Glu > α-GlcNAc2
DBATerminal α-GalNAc1
GS-Iα-Gal or α-GalNAc3
GS-IITerminal GlcNAc3
LPANANA4+
MPAα-Gal or α-GalNAc1
PNATerminal β-Gal1
SBAGalNAc > Gal1
UEA-ITerminal α(1,2)-Fuc1
WGAβ-GlcNAc > NANA1++
Succinylated WGAβ-GlcNAc1+
  • a BPA, Bauhinia purpurea agglutinin; ConA, concanavalin A; DBA, Dolichos biflorus agglutinin; GS, Griffonia simplicifolia; MPA, Maclura pomifera agglutinin; PNA, peanut agglutinin; SBA, soybean agglutinin; UEA, Ulex europaeus agglutinin.

  • b Fuc, l-fucose; Gal, d-galactose; GalNAc, N-acetyl-d-galactosamine; Glu, d-glucose; Man, d-mannose; NANA, N-acetyl-d-neuraminic acid (sialic acid). Binding specificities are as reported by the manufacturer (EY Laboratories).

  • c Buffer 1 = 0.01 M phosphate and 0.15 M NaCl (pH 7.3). Buffer 2 = 0.05 M Tris and 0.15 M NaCl (pH 7.0). Buffer 3 = 0.01 M phosphate, 0.15 M NaCl, and 0.5 mM CaCl2 (pH 7.3). Buffer 4 = 0.05 M Tris, 0.15 M NaCl, and 0.01 M CaCl2 (pH 8.0).