TABLE 2.

Insertion allele replacement

GeneA/Dahomology (bp)bNo. of colonies
5′3′CamrKansConfirmedc
xylAA1,0642592472/7
D11,3581,5264099/9
D240402499/9
crpD4040500
rpoSD40405020/2
rseBD40405011/1
yeiED404050108/8
ygaAD40405033/3
yohID40405044/4
metAD404050169/10
  • a A, amber-allele; D, deletion.

  • b That is, the length of the sequence homology on either side of the mutation.

  • c That is, the number of Kans colonies verified as replacements by PCR and sequencing of one representative clone.