TABLE 3.

Expression from the Phmg promoter is controlled by HmgRa

E. coli strainPlasmidRelevant backgroundβ-Galactosidase activity (Miller units)
UninducedPheTyr4-OH-PhPyr2,5-OH-PhAc2,5-OH-benzoate
AF141NonelacZNDbNDNDNDNDND
BMRNonePhmg-lacZ2,5202,4622,3472,2692,0172,303
BMRpQE32Phmg-lacZ1,8901,8951,9121,4251,3431,593
BMRpQ-hmgRPhmg-lacZ hmgR91514271,047198
  • a E. coli strains were grown in glycerol-containing MM in the absence (uninduced) or in the presence of 5 mM Phe, Tyr, 4-OH-PhPyr, 2,5-OH-PhAc (homogentisate), or 2,5-OH-benzoate (gentisate). β-Galactosidase activities were measured with permeabilized cells as described in Materials and Methods.

  • b ND, not detected.