TABLE 1.

Predicted ORFs and features of pPMA4326A

ORF or featureaPositionSize (aa)b%GCHomology (BLAST)cAccession no.Score/E value; aa identity (no. with identity/total [%])
A011-131143760Replication protein (repA); PtoDC3000 (pDC3000A)AAO59029775/0.0; 388/437 (88)
A021514-18019653ssDNA-binding protein (ssb); PtoDC3000AAO54791165/5e−41; 86/93 (92)
A031946-225110249Acetyltransferase; P. fluorescensZP_0008629688.6/8e−18; 46/98 (46)
MFA012630-3091462 bp56rulB; PtoDC3000 (pDC3000A)AE016855288/7e−78; 145/152 (95)
A043897-342715750Hypothetical
MFA024209-4827619 bp49Element 622 (IS-like element, no transposase)
A054895-531414060Hypothetical
A065804-612710850Conserved hypothetical; Photorhabdus luminescensNP_93152672/8e−13; 38/61 (62)
A076160-658514260Conserved hypothetical; E. coli O157:H7BAB3546577/2e−14; 42/134 (31)
A087102-746412147Hypothetical
A097637-794510355IS222 transposase orfA; Pseudomonas sp. strain ND6AAP44234178/5e−45; 94/110 (85)
A108128-879922455IS222 transposase orfB; PtoDC3000AAO56533420/1e−116; 198/223 (88)
A119541-93386853Hypothetical
A1210649-961834451Conserved hypothetical; PtoDC3000 (intergenic space)AE016856707/0.0; 47/197 (23)
A1311647-1072730750Conserved hypothetical; PtoDC3000AAO53752512/1e−162; 256/292 (87)
A1412436-1179521458Partitioning/stability (stbA); PtoPT23 (pPT23B)AAB81646362/2e−99; 188/205 (91)
A1512794-1344721849Partitioning/stability (yafB/parA); E. coli (R721)BAB12593239/8c−63; 129/217 (59)
A1613469-1380711347Partitioning/stability (yafA); E. coli (R721)BAB1259265/9e−11; 38/87 (43)
A1714012-1498932658IS1406 transposase; PtoDC3000AAO58162639/0.0; 320/325 (98)
A1815724-1533812946Hypothetical
A1916436-1596615738Pyocin S3 immunity (bip); P. aeruginosa B56394AAR02169304/1c−82; 151/156 (96)
A2016874-1652411751Transcriptional regulator (pbsX); PtoDC3000 (pDC3000B)AAO5913478.2/1e−14; 40/109 (36)
A2117139-1766617642Transcriptional regulator; PsyA2 (pPSR1)AAR02171326/4e−89; 170/175 (97)
A2217732-179477242Conserved hypothetical; PtoDC3000 (pDC3000B)AAO5913644.7/1e−04; 21/52 (40)
A2317976-1867423360Conjugal transfer (virB1); PsyA2 (pPSR1)AAR02172421/1e−117; 212/235 (90)
A2418671-1900911362Conjugal transfer (virB2); PsyA2 (pPSR1)AAR02173211/8e−55; 102/112 (91)
A2519016-1949816156Conjugal transfer (virB3); PsyA2 (pPSR1)AAR02174313/3e−85; 154/158 (97)
A2619359-2191185158Conjugal transfer (virB4); PsyA2 (pPSR1)AAR021751617/0.0; 795/848 (93)
A2721908-2259422958Conjugal transfer (virB5); PsyA2 (pPSR1)AAR02176407/1e−113; 204/228 (89)
A2822897-231307846Hypothetical
A2923141-2407331154Conjugal transfer (virB6); PsyA2 (pPSR1)AAR02177531/1e−150; 268/310 (86)
A3024141-2445510466Conjugal transfer (virB7); PsyA2 (pPSR1)AAR02178159/9e−39; 85/105 (80)
A3124492-2528026358Conjugal transfer (virB8); PsyA2 (pPSR1)AAR02179489/1e−138; 242/262 (92)
A3225270-2607927062Conjugal transfer (virB9); PsyA2 (pPSR1)AAR02180500/1e−141; 245/269 (91)
A3326204-2741540461Conjugal transfer (virB10); PsyA2 (pPSR1)AAR02181705/0.0; 355/406 (87)
A3427425-2848335362Conjugal transfer (virB11); PsyA2 (pPSR1)AAR02182620/1e−177; 294/341 (86)
A3528493-2885812260Conserved hypothetical; PgyPG4180AF346402287/6e−77; 111/118 (94)
A3629219-294858954Hypothetical
MFA0329919-3000890 bp59virD4; PsyA2 (pPSR1)AAR0218350.4/3e−06; 27/30 (90)
A3730074-3109033951Hypothetical
A3831626-3329955859Conjugal transfer (virD4); PsyA2 (pPSR1)AAR02183848/0.0; 418/555 (75)
A3933340-3370212162Killer protein (kikA); E. coli (R721)BAB12662100/1e−21; 48/97 (49)
A4033730-3589572261Conjugal transfer (traE); PsyA2 (pPSR1)AAR021841355/0.0; 684/721 (94)
A4135892-3644618558Hypothetical
A4236474-3703418761ssDNA-binding protein (ssb); PtoDC3000AAO54198360/3e−99; 175/189 (92)
MFA0437088-37285198 bp67Conserved hypothetical; P. aeruginosa PA01 (phage Pf1 PA0719)AAG0410860/2e−09; 39/66 (59)
A4337376-3809524047Partitioning/stability (stbB); PsyA2 (pPSR1)AAR02186385/1e−106; 189/238 (79)
A4438092-3866419148Conserved hypothetical; E. amylovora (pEU30)AAQ9796582/2e−15; 52/180 (28)
A4539102-3868314051Conserved hypothetical (yciA); E. coli (R721)BAB1262243:1/3e−04; 24/67 (35)
A4639444-4016924249Conjugal transfer (relaxosome component); PsyA2 (pPSR1)AAR02187445/1e−124; 222/241 (92)
A4740159-43893124558Conjugal transfer (relaxase); PsyA2 (pPSR1)AAR021882224/0.0; 1114/1250 (89)
A4844073-442285255Conserved hypothetical; PtoDC3000AAO5872479.3/5e−15; 37/53 (69)
A4944373-4480114362Transcriptional regulator (gntR); PtoDC3000 (pDC3000A)AAO59095261/2e−69; 129/142 (90)
A5045137-4605730757Conserved hypothetical; P. aeruginosa PA14AAP84187195/4e−49; 104/189 (55)
  • a MF, miscellaneous feature.

  • b Unless otherwise indicated.

  • c Abbreviations: Psy, P. syringae pv. syringae; Pto, P. syringae pv. tomato; Pma, P. syringae pv. maculicola.