TABLE 3.

lacZ::Tn allelic series

Allele no.OrientationaInsertion sitebN fragmentcActivityd
lacZ4106AS495165
lacZ4107AS726242
lacZ4108AS787262
lacZ4109AS1132377
lacZ4110AS1204401
lacZ4111AS1351450
lacZ4112AS1475491
lacZ4113AS1544514
lacZ4114AS1710570
lacZ4115AS1710570
lacZ4116AS1714571
lacZ4117AS1806602
lacZ4118AS1825608
lacZ4119AS2031677
lacZ4120AS2037679
lacZ4121AS2360786
lacZ4122AS2530843
lacZ4123AS2553851
lacZ4124AS2663887
lacZ4125S11638+
lacZ4126S726242+
lacZ4127S726242+
lacZ4128S948316
lacZ4129S1341447
lacZ4130S1400466
lacZ4131S2107702
lacZ4132S2134711
lacZ4133S2259753+/−
lacZ4134S2358786
lacZ4135S2378792++
lacZ4136S2452817
lacZ4137S2483827+
lacZ4138S2516838
lacZ4139S2628876
lacZ4140S2683894
lacZ4141S2683894
lacZ4142S2707902
lacZ4143S2707902
lacZ4144S2706902
lacZ4145S2744914
lacZ4146S2851950
lacZ4147S2929976
lacZ4148S2942980
  • a Orientation of transposon relative to Kanr transcription. AS, antisense; S, sense.

  • b Last position in the gene from the translation start before the insertion.

  • c Last amino acid residue of predicted C terminally truncated protein.

  • d Relative enzymatic activity as judged on X-Gal-containing medium. Activity: −, none; +/−, very weak; +, weak; ++, intermediate; +++, wild type.