TABLE 3.

TGS and WES activities of M. tuberculosis genes expressed in E. coli

tgs geneTGS activitya (pmol/min/mg)Optimal pHbWES activitya (pmol/min/mg)
Rv3130c (TGS1)2264.5-6.50.9
Rv3734c (TGS2)1196.5-7.29.5
Rv3234c (TGS3)936.50
Rv3088 (TGS4)796.5-7.21.8
Rv1760385.5-6.52.4
Rv2285375.5-7.23.5
Rv0221245.5-6.51.7
Rv3740c175.5-7.25.3
Rv3087145.5-6.50
Rv3371144.50.2
Rv3480c124.5-6.54
Rv3233c4.45.5-6.51.1
Rv14254.16.5-7.2ND
Rv08953.54.5-6.50.5
Rv2484c3.35.5-7.70.06
  • a The enzymatic activities were normalized for expression level as indicated in Materials and Methods. ND, not determined.

  • b A range of optimal pH is given in cases where at least 85% of activity was retained within that range. Single pH values indicate a more narrow optimal pH range.