TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
E. coli
    DH10BFmcrA D(mrr-hsdRMS-mcrBC) F80dlacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara, leu)7697 galU galK λrpsL endA1 nupGGibco/BRL Corp.
    HB101 supE44 ara14 galK2 lacY1 Δ(gpt-proA)62 rpsL20 (Strr)xyl-5 mtl-1 recA13 Δ(mcrC-mrr) HsdS-(r m)D. Ohman
C. perfringens
    NCTC 8798CPE+C. Duncan
    SM101High-frequency electroporation derivative of NCTC 8798 51
    SM120 ccpA mutant derivative of SM101This study
Plasmids
    pBluescript SK−Stratagene Corp.
    pBluescript SK+Stratagene Corp.
    pSF3Source of mob regionD. Ohman
    pJIR750 C. perfringens-E. coli shuttle vector 7
    pJIR751 C. perfringens-E. coli shuttle vector 7
    pYZ67pBluescript SK-/catPThis study
    pSM300pBluescript SK-/ermBPThis study
    pSM300MpSM300/mobilization (mob) region from pSF3This study
    pSM310pBluescript SK-/ccpAThis study
    pSM310CmpSM310/catPThis study
    pSM225pSM300M/ccpA-catPThis study
    pSM237pJIR751/cpe-gusA fusionThis study
    pSM257pJIR751/ccpAThis study
    pDOB13pJIR751/ccpAThis study