Strains, plasmids, and primers used in this study and their relevant characteristics

MaterialRelevant features or sequenceaSource or reference
    Escherichia coli MC1061Cloning host9
    Lactococcus lactis MG1363Cloning host18
    Lactobacillus plantarum
        WCFS1Wild-type for which the genome sequence is available28
        WCFS1-RRifrThis work
        NZ7109Rifr Emr; L. plantarum WCFS1 derivative containing a chromosomally located loxP-ery-loxP-cassetteThis work
    pNZ7105Apr; pUC18 derivative containing a 5′-truncated fragment of lp_3504This work
    pNZ7106Apr; pUC19 derivative containing a 5′-truncated fragment of lp_3503This work
    pNZ7107Apr; pNZ7105 derivative containing lp_3503 originating from pNZ7106This work
    pUC19lox1Apr; pUC18 derivative containing a loxP siteThis work
    pUC19lox2Apr; pUC18 derivative containing 2 loxP sites in tandem repeatThis work
    pNZ7103Apr Tetr; pUClox2 derivative containing tetR originating from pGhost8This work
    pNZ7108Apr Tetr; pNZ7107 derivative containing loxP-tetR-loxP cassette originating from pNZ7103This work
    pUC18eryApr Emr50
    pNZ7109Apr Emr; contains ery originating from pUC18ery flanked by two synthetic loxP sites and 5′-truncated fragments of lp_3503 and lp_3504 originating from pNZ7106 and pNZ7105, respectively (Fig. 1)This work
    pNZ7110Apr, pUC18 derivative containing cre and TpepN6
    pJIM2246Low-copy-cloning vector; Cmr44
    pNZ7124Cmr; pJIM2246 derivative containing cre and TpepN originating from pNZ7110This work
    pNZ7125Cmr; pNZ7124 derivative containing Tlox upstream of creThis work
    pNZ7126Cmr; pNZ7125 derivative containing PldhL1 upstream of creThis work
  • a Underlined sequences indicate restriction sites subsequently used in cloning procedures. Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Rifr, rifampicin resistant; Tetr, tetracycline resistant.