TABLE 1.

E. faecalis strains used in this study

StrainRelevant genotypeComplementation in pAT28Relevant phenotypeOrigin
V583ParentalGelE+ SprE+Clinical isolate
JML101 fsrA GelE SprE TetrpML15→V583
JML102 fsrB GelE SprE TetrpML19→V583
JML103 fsrC GelE SprE TetrpML20→V583
JML104 gelE GelE SprE TetrpML21→V583
JML105 sprE SprE TetrpML22→V583
JML106 fsrA GelE SprE Tetr SpcrpAT28→JML101
JML107 fsrA fsrA GelE+ SprE+ Tetr SpcrpML23→JML101
JML108 gelE GelE SprE Tetr SpcrpAT28→JML104
JML109 gelE gelE GelE+ SprE Tetr SpcrpML26→JML104
JML110 gelE gelE sprE GelE+ SprE+ Tetr SpcrpML27→JML104
FA2-2 fsrC GelE SprELab strain
JML111 fsrC GelE SprE SpcrpAT28→FA2-2
JML112 fsrC fsrA GelE SprE SpcrpML23→FA2-2
JML113 fsrC fsrABC GelE+ SprE+ SpcrpML24→FA2-2
JML114 fsrC fsrABC gelE sprE GelE++ SprE+ SpcrpML25→FA2-2
JML115 fsrC aphA-3 promoterGelE SprE SpcrpML28→FA2-2
JML116 fsrC aphA-3 promoter-gelEGelE++ SprE SpcrpML29→FA2-2
JML117 fsrC aphA-3 promoter-gelE sprEGelE++ SprE+ SpcrpML30→FA2-2
JML120 fsrA aphA-3 promoterGelE SprE Tetr SpcrpML28→JML101
JML121 fsrA aphA-3 promoter-gelEGelE+ SprE Tetr SpcrpML29→JML101
JML122 fsrA aphA-3 promoter-gelE sprEGelE+ SprE+ Tetr SpcrpML30→JML101