TABLE 2.

Plasmids used in this study

PlasmidDescription
p3TETIntegrational vector, Tetr derivative of p3ERM
pML15p3TET containing a 622-bp internal fragment of fsrA
pML19p3TET containing a 460-bp internal fragment of fsrB
pML20p3TET containing a 528-bp internal fragment of fsrC
pML21p3TET containing a 522-bp internal fragment of gelE
pML22p3TET containing a 444-bp internal fragment of sprE
pAT28Shuttle vector for E. coli and E. faecalis, specr
pML23pAT28 containing fsrA on a 2.0-kb EcoRI/SphI fragment
pML24pAT28 containing fsrABC on a 4.5-kb EcoRI/BglII fragment
pML25pAT28 containing fsrABC, gelE, and sprE on a 6.8-kb EcoRI/BamHI fragment
pML26pAT28 containing gelE on a 2.4-kb NheI/BamHI fragment
pML27pAT28 containing gelE and sprE on a 3.5-kb NheI/BamHI fragment
pML28pAT28 plus aphA-3 promoter on a 369-kb EcoRI/BamHI fragment
pML29pML28 containing a 1.6-kb BamHI fragment with gelE fused to the aphA-3 promoter
pML30pML28 containing a 2.8-kb BamHI fragment with gelE and sprE fused to the aphA-3 promoter